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Quantitative Differential Interference Contrast (DIC) Microscopy
Sharon King, Ariel Libertun, and Carol Cogswell
We propose to develop a high-sensitivity quantitative imaging system that should provide an alternative to fluorescence labeling for a range of contemporary live-cell investigations. Our previous theoretical and experimental investigations demonstrate that DIC has the potential to provide much more information to
biologists than is currently the case in its present commercially available form. [More]
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Quantitative phase microscopy through differential interference imaging
Sharon V. King, Ariel R. Libertun, Rafael Piestun, Chrysanthe Preza, and Carol J. Cogswell
Journal of Biomedical optics, Vol. 13, Issue 2 (2008)
Virtual Journal of Biological Physics Research (May, 2008)
Algorithms for extracting true phase from rotationally-diverse and phase-shifted DIC images
Proceedings of SPIE, 6090, January 2006
A Phase-Shifting DIC Technique for Measuring 3D Phase Objects: Experimental Verification
Proceedings of SPIE, 5324, 191-196, January 2004
Three dimensional imaging with rotating point spread functions
Sri Rama Prasanna Pavani and Rafael Piestun
We design efficient diffractive optical elements to generate rotating point spread functions for incoherent three-dimensional computational imaging systems. Higher diffraction efficiency is important for increasing signal-to-noise and accuracy. [More]
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Tracking of fluorescent microspheres in three dimensions using an efficient rotating point spread function
Sri Rama Prasanna Pavani and Rafael Piestun
(in preparation)
3D localization with nanometer accuracy using high-efficiency rotating point spread functions
Sri Rama Prasanna Pavani, Adam Greengard, and Rafael Piestun
(to be submitted)
3D localization of fluorescent microparticles using a rotating point spread function
Sri Rama Prasanna Pavani and Rafael Piestun
OSA Frontiers in Optics (FiO), Rochester, USA, Oct. 2008 (submitted)
High-efficiency rotating point spread functions
Sri Rama Prasanna Pavani and Rafael Piestun
Optics Express, vol. 16 issue 5, 3484-3489 (2008)
Virtual Journal for Biomedical Optics, Vol. 3 Issue 4 (2008)
Passive 3D imaging with quasi-rotating PSFs
Sri Rama Prasanna Pavani and Rafael Piestun
OSA Digital Holography and 3D imaging, St. Petersburg, USA, Mar. 2008
Efficient rotating point spread functions for 3D imaging
Sri Rama Prasanna Pavani and Rafael Piestun
OSA Frontiers in Optics, San Jose, USA, Sept. 2007
Efficient diffractive optical elements for depth from diffracted rotation systems
Sri Rama Prasanna Pavani and Rafael Piestun
OSA Computational Optical Sensing and Imaging (COSI), Vancouver, Canada, June 2007
Structured Illumination Quantitative phase microscopy
Sri Rama Prasanna Pavani, Ariel Libertun, Sharon King, and Carol Cogswell
Phase only objects like transparent cells do not absorb light, and therefore are almost invisible to traditional bright field transmission microscopes having square law intensity detectors. We propose two simple additions to a traditional bright field microscope for accomplishing quantitative phase imaging. [More]
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Quantitative structured-illumination phase microscopy
Sri Rama Prasanna Pavani, Ariel R. Libertun, Sharon V. King, and Carol J. Cogswell
Applied Optics, vol. 47 issue 1, 15-24 (2008)
Virtual Journal for Biomedical Optics, Vol. 3 Issue 2 (2008)
QSIP: Phase imaging made possible in a bright field microscope
Sri Rama Prasanna Pavani, Ariel R. Libertun, Sharon V. King, and Carol J. Cogswell
SPIE Photonics West (BIOS), San Jose, USA, 2008
Quantitative phase estimation with a brightfield microscope
Sri Rama Prasanna Pavani, Ariel R. Libertun, and Carol J. Cogswell
OSA Frontiers in Optics, San Jose, USA, Sept. 2007
Structured-illumination quantitative phase microscopy
Sri Rama Prasanna Pavani, Ariel R. Libertun and Carol J. Cogswell
OSA Computational Optical Sensing and Imaging (COSI), Vancouver, Canada, June 2007
Wavefront Coding
Sharon King, Sri Rama Prasanna Pavani, Carol Cogswell, and W. Thomas Cathey
We have designed an optical/digital system that delivers near diffraction limited imaging performance with a large depth of field. This system is the standard incoherent optical system modified by a phase mask, with digital processing of the resulting intermediate image. The phase mask alters or codes the received incoherent wavefront in such a way that the PSF and OTF do not appreciably change as a function of misfocus. Focus independent digital filtering of the intermediate image is used to produce a combined optical/digital system that has a nearly diffraction limited PSF. [More]
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NSF Disclaimer: This material is based upon work supported by the National Science Foundation under Grant No. 0455408. Any opinions, findings, and conclusions or recommendations expressed in this material are those of the author(s) and do not necessarily reflect the views of the National Science Foundation.
The Micro Optical - Imaging Systems Laboratory is affiliated with the
Optoelectronics Computing Systems Center and the
Department of Electrical and Computer Engineering
at the 
University of Colorado-Boulder.
webmaster: pavani@colorado.edu